An efficient method of DNA extraction from coconut leaves. In: Agrotrópica v. 11 p. 35-38, 1999.

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dc.contributor.authorUpadhyay, Anuradha-
dc.contributor.authorParthasarathy, V. A.-
dc.contributor.authorSeema, G.-
dc.contributor.authorKarun, Anitha-
dc.date.accessioned2023-10-19T13:34:22Z-
dc.date.available2023-10-19T13:34:22Z-
dc.date.issued1999-
dc.identifier.urihttp://192.168.3.118:8080/handle/1/1702-
dc.description.abstractA protocol for extraction of good quality , high molecular weight genomic DNA from young leaves of coconut was standardized using two detergents viz. sodium dodecyl sulphate (SDS) and hexadecyl trimethyl ammonium bromide (CTAB) at different concentrations; at pH 8.0 and pH 9.0. SDS (1 %) at pH 8.0 was found to yield good quantity of high quality DNA. The extracted DNA was amenable to PCR and restriction digestion.pt_BR
dc.language.isoenpt_BR
dc.publisherMinistério da Agricultura, Pecuária e Abastecimento (MAPA)pt_BR
dc.subjectCOCOpt_BR
dc.subjectPROTOCOLOpt_BR
dc.subjectDNApt_BR
dc.subjectPCRpt_BR
dc.subjectRFLPpt_BR
dc.titleAn efficient method of DNA extraction from coconut leaves. In: Agrotrópica v. 11 p. 35-38, 1999.pt_BR
dc.typeArtigopt_BR
dc.contributor.copyrightMinistério da Agricultura, Pecuária e Abastecimento (MAPA)-
Aparece nas coleções:Revista Agrotrópica
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