An efficient method of DNA extraction from coconut leaves. In: Agrotrópica v. 11 p. 35-38, 1999.
| Título: | An efficient method of DNA extraction from coconut leaves. In: Agrotrópica v. 11 p. 35-38, 1999. |
| Autor(es): | Upadhyay, Anuradha Parthasarathy, V. A. Seema, G. Karun, Anitha |
| Editor: | Ministério da Agricultura, Pecuária e Abastecimento (MAPA) |
| Idioma: | en |
| Tipo: | Artigo |
| Data: | 1999 |
| Resumo: | A protocol for extraction of good quality , high molecular weight genomic DNA from young leaves of coconut was standardized using two detergents viz. sodium dodecyl sulphate (SDS) and hexadecyl trimethyl ammonium bromide (CTAB) at different concentrations; at pH 8.0 and pH 9.0. SDS (1 %) at pH 8.0 was found to yield good quantity of high quality DNA. The extracted DNA was amenable to PCR and restriction digestion. |
| Palavras-chave: | COCO; PROTOCOLO; DNA; PCR; RFLP |
| URI: | http://192.168.3.118:8080/handle/1/1702 |
| Aparece nas coleções: | Revista Agrotrópica |
Arquivos associados a este item:
| Arquivo | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| br2019000894.pdf | 13,78 MB | Adobe PDF | Visualizar/Abrir |
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