Optimal PCR parameters for RAPD analysis in cocunut. In: Agrotrópica v. 13 p. 87-90, 2001.

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dc.contributor.authorUpadhyay, Anuradha-
dc.contributor.authorPrakash, K.-
dc.contributor.authorJose, Jeslin-
dc.contributor.authorParthasarathy, V. A.-
dc.date.accessioned2023-10-16T19:09:36Z-
dc.date.available2023-10-16T19:09:36Z-
dc.date.issued2001-
dc.identifier.urihttp://192.168.3.118:8080/handle/1/1669-
dc.description.abstractCentral Plantation Crops Research Institute, Kasaragod, India, maintains a large collection of coconut germplasm. Use of molecular markers including RAPD markers is underway to characterize coconut germplasm. A reproducible PCR protocol is prerequisite for usefulness of RAPD markers in germplasm analysis. Varying concentrations of primer, dNTP, enzyme, MgCl2 were used to find the optimum reaction conditions. MgCl2 at 4-4.5 µM, 100 µM of each dNTP, 1.5 units of enzyme, 20-25 pmol primer and annealing temperature of 55°C and above gave highly reproducible PCR products.pt_BR
dc.language.isoenpt_BR
dc.publisherMinistério da Agricultura, Pecuária e Abastecimento (MAPA)pt_BR
dc.subjectCOQUEIROpt_BR
dc.subjectCOMPONENTES DE PCRpt_BR
dc.subjectRAPDpt_BR
dc.titleOptimal PCR parameters for RAPD analysis in cocunut. In: Agrotrópica v. 13 p. 87-90, 2001.pt_BR
dc.typeArtigopt_BR
dc.contributor.copyrightMinistério da Agricultura, Pecuária e Abastecimento (MAPA)-
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