Optimal PCR parameters for RAPD analysis in cocunut. In: Agrotrópica v. 13 p. 87-90, 2001.

Abstract

Central Plantation Crops Research Institute, Kasaragod, India, maintains a large collection of coconut germplasm. Use of molecular markers including RAPD markers is underway to characterize coconut germplasm. A reproducible PCR protocol is prerequisite for usefulness of RAPD markers in germplasm analysis. Varying concentrations of primer, dNTP, enzyme, MgCl2 were used to find the optimum reaction conditions. MgCl2 at 4-4.5 µM, 100 µM of each dNTP, 1.5 units of enzyme, 20-25 pmol primer and annealing temperature of 55°C and above gave highly reproducible PCR products.