Regeneration of isolated protoplasts of Theobroma cacao. In: Agrotrópica v. 10 p. 135-138, 1998.

Abstract

Conditions were established for efficient isolation and regeneration of protoplasts of cocoa (Theabrama cacao). Protoplasts were obtained from in vitro cell suspension, established from callus derived from leaves and buds. The mix cellulase 4% and e macerocyme 2% yielded high number of protoplasts with a high efficiency of isolation and viability. Regeneration of protoplasts in medium containing 7-8% mannitol as osmoticum presented high cell division.