Temporal genetic characterization of isolates of Moniliophthora perniciosa in southeast of Bahia, Brazil. In: Agrotrópica v. 24 p. 169-178, 2012.

Abstract

Random amplified polymorphism DNA (RAPD) markers were used to characterize temporal genetic variability of Moniliophthora perniciosa populations in Bahia, Brazil, over four consecutive years (2001 to 2004). Five primers (OPH20, OPH13, OPI19, OPI14, and OPL7) showing reproducible polymorphism and clear banding patterns were used to amplify the genomic DNA of 89 isolates, generating a total of 42 bands. The matrix of RAPD data revealed the occurrence of genetic differentiation among years. Most of the genetic diversity was attributed to the differences between 2004 and the remaining years. The principal component analysis and AMOVA (P < 0.001) confirmed the presence of populations related to the year of collection. Overall, isolates from the same year shared the same coordinates. Weir and Cockerham’s theta was used to estimate the degree of genetic differentiation between populations. The 2001 and 2002, and 2002 and 2003 populations were the most similar (è = 0.18 and è = 0.11; respectively, p<0.001), while the 2002 and 2004 populations differentiated the most (è = 0.51, p<0.001). The shift in the genetic composition of the populations studied seems to be correlated with the the increase of planting areas with Scavina descendant genotypes.